We occasionally run into what appear to be transcriptional/translational stalling issues and I am interested to learn your preferred backbones for bacterial protein expression. Please share your favorite options and let me know if you have a good reason for that choice. Thanks!
In PEL we are most commonly using our own pET-derived plasmids:
pDest-527 (N-term His6) https://www.addgene.org/11518/
pDest-566 (N-term His6-MBP) https://www.addgene.org/11517/