We occasionally run into what appear to be transcriptional/translational stalling issues and I am interested to learn your preferred baculovirus backbones. Please share your favorite options and let me know if you have a good reason for that choice. Thanks!
In PEL we are most commonly using our own pFastBac-derived bacmids:
pDest-635 (N-term His6) https://www.addgene.org/159582/
pDest-636 (N-term His6-MBP) https://www.addgene.org/159574/