Protein Interest Group

We have a generally similar process to what Carter described, when exploring Ecoli for recombinant expression needs. We small-scale (plate-based) screen at 18C post induction overnight, in TB and auto-induction media (2 data points for each clone). We induce with 100uM IPTG. From time to time, we ex...

In our labs, we primarily use Nickel ProBond (Invitrogen/Thermo). We primarily utilize batch bind/elution for primary affinity capture (this includes small-scale plate-based expression/purification as well as multiliter volume prep scale). We primarily do gravity flow for Ni-reverse clean up of prot...